Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [1521]. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Knockdown Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [1521].

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody [1521] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IDH1 protein stained by IDH1 antibody [1521] diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 10 % SDS-PAGE IDH1 antibody [1521] dilution: 1:1000

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody [1521] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) [NBP2-43692] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, RM, Sh
• Applications: WB, ICC/IF, IP, KD, KO
• Clone: 1521
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Isocitrate Dehydrogenase 1/IDH1. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%), Bovine (95%), Sheep (95%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDH1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:1000
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (1521) - Azide and BSA Free

• Cytosolic NADP-isocitrate dehydrogenase
• EC 1.1.1.42
• IDCD
• IDH
• IDH1
• IDP
• IDPC
• isocitrate dehydrogenase [NADP] cytoplasmic
• isocitrate dehydrogenase 1 (NADP+), soluble
• Isocitrate Dehydrogenase 1
• NADP(+)-specific ICDH
• NADP-dependent isocitrate dehydrogenase, cytosolic
• NADP-dependent isocitrate dehydrogenase, peroxisomal
• Oxalosuccinate decarboxylase
• PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH1