CEBP alpha Antibody

CEBP a was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CEBP a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7094) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

Development of endothelial and mesenchymal like cells and maturation of hepatic endoderm.(a) CD34 and CD93 were detected in endothelial progenitor-like cells on day 7. CEBP alpha +marks the hepatic endoderm in the background (scale bars, 200 μm). (b) Development of endothelial-like cells (arrows indicate CD31). CD34 is present in the hepatocyte-like cell fraction (CEBP alpha +, CD146−) and in the endothelial-like cells (CEBP alpha −, CD146+). (c) CD51+, NES+, PDGFR alpha + mesenchymal stem cell-like cells develop in conjunction with hepatoblasts. (d) AAT and CEBP alpha on day 8 and (e) 10 show maturation of hepatic endoderm. (f) Upregulation of AAT and fibrinogen (Fib) synthesis (ELISA). Data are mean±s.e.m., n=3 per group. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26732624), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CEBP alpha Antibody Summary

• Immunogen: E. coli-derived recombinant human CEBP alpha
  Met1-Ala124
  Accession # P49715
• Specificity: Detects human CEBP alpha in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CEBP beta , rhCEBP gamma , rhCEBP δ, rhCEBP zeta , and rhCEBP epsilon is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CEBPA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEBP alpha Antibody

• C/EBP alpha
• C/EBP-alpha
• CCAAT/enhancer binding protein (C/EBP), alpha
• CCAAT/enhancer-binding protein alpha
• CEBP alpha
• CEBP
• CEBPA

Background

CEBP alpha (CCAAT/Enhancer-Binding Protein; also C/EBP) is a 42-43 kDa member of the C/EBP subfamily, bZIP family of proteins. It is expressed in multiple cell types, particularly those that are fully differentiated, and includes hepatocytes, skeletal muscle cells, adipocytes, intestinal epithelium and proliferating macrophages. CEBP alpha acts as a transcriptional activator for a large and diverse number of genes, including glycogen synthase, GLUT4, and G-CSFR, and participates in both cell cycle progression and differentiation. Human CEBP alpha is 358 amino acids (aa) in length). It contains one transactivation domain (aa 1-96), poly-Gly segment (aa 99-104), a poly-Pro motif (aa 183-189), a bZIP dimerization region (aa 281-334), and an overlapping DNA-binding Leu-zipper domain (aa 317-345). CEBP alpha forms both homodimers, and heterodimers with other subfamily members. One alternative start site at Met120 generates a low activity 30-32 kDa isoform variant. There is also a utilized phosphorylation site at Ser21 that regulates activity. Over aa 1-124, human CEBP alpha shares 93% aa identity with mouse CEBP alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEBP alpha Antibody (AF7094)(1)

Publication using AF7094

• JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022-05-25;0(0):. 5/25/2022 [PMID: 35614216]

Bioinformatics

• Gene Symbol: CEBPA
• Uniprot:
  • P49715(Human)