Islet-1 Antibody (1H9)

Images

 
Western Blot: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).
Immunocytochemistry/ Immunofluorescence: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
1H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Islet-1 Antibody (1H9) Summary

Immunogen
Purified recombinant fragment of human ISL1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ISL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Adrenal Lysate (NB820-59280)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Islet-1 Antibody (1H9)

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

ISL1 (ISL1 transcription factor, LIM/homeodomain) is a member of the LIM/homeodomain family of transcription factors. It binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. It is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Islet-1 expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ISL1
Entrez
Uniprot