Irisin/FNDC5 Antibody (1056111) [Unconjugated]

Irisin/FNDC5 was detected in immersion fixed CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94201) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1056111
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Irisin/FNDC5 Antibody (1056111) [Unconjugated] Summary

• Immunogen: Synthetic peptide covering the C-term of the Irisin peptide sequences of the FNDC5 gene.
  
  Glu97-Glu143
  Accession # Q8NAU1
• Specificity: Detects Irisin peptide in direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Irisin/FNDC5 Antibody (1056111) [Unconjugated]

• fibronectin type III domain containing 5
• fibronectin type III domain-containing protein 5
• FNDC5
• FRCP2
• FRCP2Fibronectin type III repeat-containing protein 2
• Irisin

Background

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

1. Bostrom, P. et al. (2012) Nature 481:463.
2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
4. Teufel, A. et al. (2002) Gene 297:79.
5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
10. Dun, S.L. et al. (2013) Neuroscience 240:155.
11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
13. Moon, H.S. et al. (2013) Metabolism 62:1131.
14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8NAU1()