IRF6 Antibody

Images

 
Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IRF6 Antibody Summary

Immunogen
Peptide with sequence C-TPSMQLPPALPPQ corresponding to C-Terminus according to NP_006138.1.
Epitope
C-TPSMQLPPALPPQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 53.1 kDa band according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows strong nuclear staining in DCT and glomerulus.
Publications
Read Publications using
NBP1-51911 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25358290).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRF6 Antibody

  • interferon regulatory factor 6
  • IRF6
  • IRF-6
  • LPS
  • OFC6
  • OFC6PPS
  • PIT
  • PPS
  • Van der Woude syndrome
  • VWS
  • VWS1

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF6 Antibody (NBP1-51911)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP1-51911 Applications Species
Li C, Ying W, Huang Z et al. IRF6 regulates alternative activation by suppressing PPARg in male murine macrophages. Endocrinology. 2017 Jun 22 [PMID: 28645193]
Hu L, Liu J, Li Z et al. TGFB3 Regulates Periderm Removal through delta-np63 in the Developing Palate J. Cell. Physiol. 2014 Oct 30 [PMID: 25358290] (WB, Mouse)

Details:
IRF6 antibody used for WB on lysates of medial edge epithelial cells collected from WT mouse at 14.25 dpc subjected to exogenous recombinant rTGF beta 3 (5ng/mL) treatment. Figure 6 i and ii shows the time and concentration dependent response of cells to rTGF beta 3.
WB Mouse
Botti E, Spallone G, Moretti F et al. Developmental factor IRF6 exhibits tumor suppressor activity in squamous cell carcinomas. Proc Natl Acad Sci U S A. 2011 Aug 16 [PMID: 21807998] (ChIP)

Details:
ChiP and ChiP-seq analysis (primary human keratinocytes): Figs 3, 4.
ChIP
Moretti F, Marinari B, Lo Iacono N et al. A regulatory feedback loop involving p63 and IRF6 links the pathogenesis of 2 genetically different human ectodermal dysplasias. J Clin Invest. 2010 May [PMID: 20424325] (WB)

Details:
WB (primary human keratinocytes): Figs 1F, 4B (keratinocytes were transfected with IRF6 or control siRNA). IF (human skin cells from biopsies growing on chamber slides, fixed with 4% PFA and permeabilized with 0.2% Triton), Fig S2. Note: The specificity o
WB
Kondo S, Schutte BC, Richardson RJ et al. Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes. Nat Genet 2002 Oct [PMID: 12219090]

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Bioinformatics

Gene Symbol IRF6
Entrez
Uniprot