IRF5 Antibody (SD203-07)

Western Blot: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of IRF5 on THP-1 cells lysates using anti-IRF5 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. Counter stained with hematoxylin.

Flow Cytometry: IRF5 Antibody (SD203-07) [NBP2-67742] - Analysis of Hela cells with IRF5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: IRF5 Antibody (SD203-07) [NBP2-67742] - Staining IRF5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clone: SD203-07
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRF5 Antibody (SD203-07) Summary

• Description: Novus Biologicals Rabbit IRF5 Antibody (SD203-07) (NBP2-67742) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human IRF5 aa 1-200. (SwissProt: Q13568 Human; SwissProt: P56477 Mouse; Unigene:203787 Rat)
• Localization: Nucleus, Cytoplasm.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRF5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Simple Western 1:50
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IRF5 Antibody (SD203-07)

• IBD14
• interferon regulatory factor 5
• IRF5
• IRF-5
• SLEB10

Background

IRF5 encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF5