IRF3 Antibody (PCRP-IRF3-1E6) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Immunofluorescence Analysis of PFA-fixed HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (green). Counterstain is phalloidin.

Flow Cytometry: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Flow Cytometric Analysis of PFA-fixed HeLa cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).

Protein Array: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MA
• Clone: PCRP-IRF3-1E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IRF3 Antibody (PCRP-IRF3-1E6) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07529).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human IRF3 protein (Uniprot: Q14653)
• Localization: Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for IRF3 Antibody (PCRP-IRF3-1E6) - Azide and BSA Free

• interferon regulatory factor 3
• IRF3
• IRF-3

Background

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF3