IRF3 Antibody (OTI2G3)

Western Blot: IRF3 Antibody (2G3) [NBP1-47812] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.

Immunocytochemistry/Immunofluorescence: IRF3 Antibody (2G3) [NBP1-47812] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF3.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded pancreas tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded breast tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded colon tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded endometrium tissue using anti-IRF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IRF3 Antibody (2G3) [NBP1-47812] - Staining of paraffin-embedded Kidney tissue using anti-IRF3 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Single-Cell Western
• Clone: OTI2G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRF3 Antibody (OTI2G3) Summary

• Description: Novus Biologicals Mouse IRF3 Antibody (OTI2G3) (NBP1-47812) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human IRF3 expression vector
• Specificity: This antibody is specific for Homo sapiens interferon regulatory factor 3 (IRF3).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Single Cell Western 100 ug/ml
  • Western Blot 1:2000
• Theoretical MW: 47.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRF3 Antibody (OTI2G3)

• interferon regulatory factor 3
• IRF3
• IRF-3

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF3
• Entrez:
  • 3661(Human)