IRF3 Antibody (3F10)

Images

 
Western Blot: IRF3 Antibody (3F10) [NBP1-04308] - PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ...read more
Immunocytochemistry/ Immunofluorescence: IRF3 Antibody (3F10) [NBP1-04308] - Human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
Immunoprecipitation: IRF3 Antibody (3F10) [NBP1-04308] - Immunoprecipitation (IP) was performed in HeLa cell with IRF-3 mAb (1ug/ml). Proteins were analyzed by IRF-3 antibody and ECL detection system. (a) Normal IgG, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
3F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

IRF3 Antibody (3F10) Summary

Immunogen
Recombinant human IRF-3(108-166aa) purified from E. coli
Specificity
Anti-human IRF-3 mAb, clone 3F10, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF3 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IRF3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IRF3 Antibody (3F10)

  • interferon regulatory factor 3
  • IRF3
  • IRF-3

Background

Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRF3 Antibody (NBP1-04308) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-04308AF405
Alexa Fluor 488 Labeled NBP1-04308AF488
Alexa Fluor 647 Labeled NBP1-04308AF647
Alexa Fluor 700 Labeled NBP1-04308AF700
DyLight 350 Labeled NBP1-04308UV
DyLight 405 Labeled NBP1-04308V
DyLight 488 Labeled NBP1-04308G
DyLight 680 Labeled NBP1-04308FR
DyLight 755 Labeled NBP1-04308IR

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Bioinformatics Tool for IRF3 Antibody (NBP1-04308)

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Research Areas for IRF3 Antibody (NBP1-04308)

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Blogs on IRF3.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Bioinformatics

Gene Symbol IRF3
Entrez
Uniprot