IRAK4 Antibody (413613) [Unconjugated]

K562 human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Jurkat human cell line was stained with Rat Anti-Human IRAK Monoclonal Antibody (Catalog # MAB39191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

IRAK4 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human IRAK4 Monoclonal Antibody (Catalog # MAB39191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 413613
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IRAK4 Antibody (413613) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IRAK4
  Met1-Ser460
  Accession # Q9NWZ3
• Specificity: Detects human IRAK4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRAK4 Antibody (413613) [Unconjugated]

• EC 2.7.11
• EC 2.7.11.1
• interleukin-1 receptor-associated kinase 4
• IPD1
• IRAK4
• IRAK-4
• NY-REN-64
• REN64
• Renal carcinoma antigen NY-REN-64

Background

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9NWZ3()