IRAK2 Antibody

Western Blot: IRAK2 Antibody [NB100-56447] - Analysis of IRAK-2 in 15 ugs of Jurkat cell lysate using this antibody at 1 ug/ml.

Western Blot: IRAK2 Antibody [NB100-56447] - Analysis of IRAK-2 in A) human K562 and B) mouse RAW lysate using this antibody at 3 ug/ml.

Product Discontinued

IRAK2 Antibody Summary

• Immunogen: This antibody was developed against a synthetic peptide of human IRAK-2.
• Specificity: In Jurkat, a 67 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IRAK2 Antibody

• interleukin-1 receptor associated kinase-2
• interleukin-1 receptor-associated kinase 2
• interleukin-1 receptor-associated kinase-like 2
• IRAK2
• IRAK-2
• MGC150550

Background

The binding of interleukin-1 (IL-1) to its receptor IL-1RI initiates a signaling cascade that results in the activation of NF-kB. Recently protein kinases designated IRAKs (IL-1 receptor-associated kinases) have been identified and designated as IRAK-1, IRAK-2 and IRAK-M. IRAK-2 is a serine/threonine protein kinase. The primary amino acid sequence of IRAK-2 shares similarity with that of Pelle, a protein kinase that is essential for the activation of a NF-kB homolog in Drosophila. IRAK (IL-1 receptor kinase) and mediates NF-kB activation by members of IL-1 family including IL-1 and a novel cytokine IL-18. Disruption of IRAK expression by anti-sense oligonucleotides inhibits IL-1 and IL-18-induced activation of NF-kB and activator protein, AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK2 Antibody (NB100-56447)(1)

Publication using NB100-56447

• Kim SJ, Cha JY, Kang HS et al. Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages. BMB Rep. 2016-05-01 [PMID: 26615974] (WB, Mouse)

Bioinformatics

• Gene Symbol: IRAK2
• Entrez:
  • 3656(Human)
• Uniprot:
  • O43187()