Involucrin Antibody (SY5) - BSA Free

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Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. ...read more
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).

Product Details

Summary
Reactivity Hu, Po, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
SY5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Involucrin Antibody (SY5) - BSA Free Summary

Immunogen
Purified involucrin from human keratinocytes.
Epitope
The epitope maps between codon 421-568 of human involucrin.
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IVL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1.0 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 0.1-0.2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
IHC: Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 degrees Celsius. Note than enzyme digestion is better than citrate buffer based epitope unmasking. This antibody is CyTOF ready.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MCF-7 Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-2727 in the following applications:

Publications
Read Publications using NB100-2727.

Reactivity Notes

There is weak reactivity with Canine, but it does not react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Notes

Positive control(s): MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Alternate Names for Involucrin Antibody (SY5) - BSA Free

  • involucrin

Background

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Involucrin Antibody (NB100-2727)(2)

Review for Involucrin Antibody (NB100-2727) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2727:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Involucrin NB100-2727
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4
reviewed by:
Adam Richardson
WB Human 04/25/2018
View

Summary

ApplicationWestern Blot
Sample TestedCultured Human Keratinocytes,Human keratinocytes
SpeciesHuman
LotCRT/15/31

Comments

CommentsBlock in 5% nonfat dry milk in TBST incubated at RT for 1 hr
1:500 in TBST, overnight at 4C

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

FAQs for Involucrin Antibody (NB100-2727) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
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1
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Adam Richardson
04/25/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol IVL
Entrez