Involucrin Antibody (SY5)

Images

 
Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. ...read more
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).

Product Details

Summary
Reactivity Hu, Po, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
SY5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Involucrin Antibody (SY5) Summary

Immunogen
Purified involucrin from human keratinocytes.
Epitope
The epitope maps between codon 421-568 of human involucrin.
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IVL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 0.5 - 1.0 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 0.1-0.2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
Application Notes
IHC: Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 degrees Celsius. Note than enzyme digestion is better than citrate buffer based epitope unmasking. This antibody is CyTOF ready.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MCF-7 Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-2727 in the following applications:

Publications
Read Publication using NB100-2727.

Reactivity Notes

There is weak reactivity with Canine, but it does not react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Notes

Positive control(s): MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Alternate Names for Involucrin Antibody (SY5)

  • involucrin

Background

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready

Publications for Involucrin Antibody (NB100-2727)(1)

Review for Involucrin Antibody (NB100-2727) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2727:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Involucrin NB100-2727
Enlarge
reviewed by:
Anonymous
WB Human 04/25/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman keratinocytes,Cultured Human Keratinocytes
SpeciesHuman
LotCRT/15/31

Comments

CommentsBlock in 5% nonfat dry milk in TBST incubated at RT for 1 hr
1:500 in TBST, overnight at 4C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Involucrin Antibody (NB100-2727) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2727AF350
Alexa Fluor 405 NB100-2727AF405
Alexa Fluor 488 NB100-2727AF488
Alexa Fluor 532 NB100-2727AF532
Alexa Fluor 594 NB100-2727AF594
Alexa Fluor 647 NB100-2727AF647
Alexa Fluor 700 NB100-2727AF700
Alexa Fluor 750 NB100-2727AF750
Allophycocyanin NB100-2727APC
Allophycocyanin/Cy7 NB100-2727APCCY7
DyLight 350 NB100-2727UV
DyLight 405 NB100-2727V
DyLight 488 NB100-2727G
DyLight 550 NB100-2727R
DyLight 594 NB100-2727DL594
DyLight 650 NB100-2727C
DyLight 680 NB100-2727FR
DyLight 755 NB100-2727IR
FITC NB100-2727F
Janelia Fluor 549 NB100-2727JF549
Janelia Fluor 646 NB100-2727JF646
PE NB100-2727PE
PE/Atto594 NB100-2727PEATT594
PE/Cy5.5 NB100-2727PECY55
PE/Cy7 NB100-2727PECY7
PerCP NB100-2727PCP

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
04/25/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol IVL
Entrez