Intra Acrosomal Protein Antibody (Ds-2) - BSA Free

• Reactivity: Ca
• Applications: WB, ICC/IF
• Clone: Ds-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Intra Acrosomal Protein Antibody (Ds-2) - BSA Free Summary

• Description: Novus Biologicals Mouse Intra Acrosomal Protein Antibody (Ds-2) - BSA Free (NB500-436) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
• Specificity: The antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACRV1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: Immunocytochemistry: Membrane permeabilization (acetone) is essential.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Intra Acrosomal Protein Antibody (Ds-2) - BSA Free

• acrosomal protein SP-10
• acrosomal vesicle protein 1D11S4365
• SP-10
• SPACA2
• sperm protein 10

Background

The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of canine sperms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACRV1