SPACA3 Antibody

Images

 
Western Blot: SPACA3 Antibody [NBP1-89136] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed ...read more
Immunocytochemistry/ Immunofluorescence: SPACA3 Antibody [NBP1-89136] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: SPACA3 Antibody [NBP1-89136] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferus ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SPACA3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:TNNGIFQINSRRWCSNLTPNVPNVCRMYCSDLLNPNLKDTVICAMKITQEPQGLGYWEAWRHHCQGKDLTEWVDGCDF
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPACA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SPACA3 Protein (NBP1-89136PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SPACA3 Antibody

  • CT54ALLP17Cancer/testis antigen 54
  • LYC3Sperm protein reactive with ASA
  • Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17
  • Lysozyme-like protein 3
  • lysozyme-like sperm-specific secretory protein ALLP17
  • LYZL31700025M08Rik
  • SLLP1MGC119058
  • sperm acrosome associated 3
  • sperm acrosome membrane-associated protein 3
  • sperm lysozyme like protein 1
  • Sperm lysozyme-like protein 1
  • Sperm protein reactive with antisperm antibodies
  • SPRASA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SPACA3 Antibody (NBP1-89136)

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Diseases for SPACA3 Antibody (NBP1-89136)

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Bioinformatics

Gene Symbol SPACA3