Intelectin-1/Omentin Antibody (746420)

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intelectin‑1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin‑1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Microbiota suppresses transcriptional nutrient stress response and activates mTOR signaling. A) Overlay of nutrient starvation stress response transcription factor genes upon gene expression volcano plot of all GF and SPF epithelial cells. B) Violin plot of Atf3 and Jun expression across GF and SPF epithelial cells. C) Violin plot of Fos expression across GF and SPF epithelial cells and quantification and representative image of Fos in situ hybridization within GF and SPF jejunal/ileal small intestinal crypts. D) Violin plots and E) UMAP visualization of Rps6kb1 and Rps6 epithelial subsets in GF and SPF epithelial cells. *, p 0.75 Log2 fold change. F) pS6 staining and quantification of jejunal/ileal small intestinal crypts from GF and SPF mice. G) Immunofluorescence co-staining of BrdU, pS6, and Itln1 in GF and SPF jejunal/ileal small intestinal crypts. Arrows indicate co-expression of Itln1 and pS6. Representative images shown (n = 4–6). Each point represents the mean quantification 20 crypts from one mouse (n = 4–6). Stains were quantified using CellProfiler. Bars represent mean ± SEM. **, p 0.75 Log2 fold change. F) pS6 staining and quantification of jejunal/ileal small intestinal crypts from GF and SPF mice. G) Immunofluorescence co-staining of BrdU, pS6, and Itln1 in GF and SPF jejunal/ileal small intestinal crypts. Arrows indicate co-expression of Itln1 and pS6. Representative images shown (n = 4–6). Each point represents the mean quantification 20 crypts from one mouse (n = 4–6). Stains were quantified using CellProfiler. Bars represent mean ± SEM. **, p < .005 by two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35939622), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 746420
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-1/Omentin Antibody (746420) Summary

• Immunogen: E. coli-derived recombinant mouse Intelectin‑1/Omentin
  Ala20-Ser298
  Accession # O88310
• Specificity: Detects mouse Intelectin‑1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ITLN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-1/Omentin Antibody (746420)

• Endothelial lectin HL-1
• FLJ20022
• Galactofuranose-binding lectin
• hIntL
• HL-1
• intelectin 1 (galactofuranose binding)
• Intelectin1
• Intelectin-1
• INTLHL1
• ITLN1
• Itlna
• ITLNITLN-1
• LFR
• LFRIntestinal lactoferrin receptor
• Omentin
• Omentin-1

Background

Intelectin (endothelial lectin; also ITLN-1 and omentin) is a 34 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed in intestinal and airway epithelium and appears to participate in insulin signaling and microbe recognition. Intelectin-1 is upregulated in asthma and contributes to allergic airway inflammation. The mouse Intelectin-1 preproprecursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature segment (aa 20-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. Soluble Intelectin-1 can be released from omental adipose tissue and malignant pleural mesothelioma. One fibrinogen-related domain exists in the mature molecule (aa 32-251). Human Intelectin-1 exists as a disulfide-linked glycosylated homotrimer; mouse is unglycosylated and a monomer. Mature mouse Intelectin-1 is 82% aa identical to human Intelectin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Intelectin-1/Omentin Antibody (MAB8074)(4)

Publications using MAB8074

• Goyal, S;Guo, CX;Singh, O;Ranger, A;Harrigan, CF;Meade, J;Luchak, A;Tsang, DK;Gaisano, HY;Gao, N;Yuzwa, SA;Wrana, JL;Philpott, DJ;Gray-Owen, SD;Girardin, SE; Bacterial ADP-heptose triggers stem cell regeneration in the intestinal epithelium following injury Cell stem cell 2025-08-07 [PMID: 40651470] (Immunohistochemistry, Transgenic Mouse)
• M Sigal, MDM Reinés, S Müllerke, C Fischer, M Kapalczyns, H Berger, ERM Bakker, HJ Mollenkopf, ME Rothenberg, B Wiedenmann, S Sauer, TF Meyer R-spondin-3 induces secretory, antimicrobial Lgr5+ cells in the stomach Nat. Cell Biol., 2019-06-24;21(7):812-823. 2019-06-24 [PMID: 31235935] (Immunohistochemistry, Western Blot, Bacteria - Helicobacter pylori, Mouse, Transgenic Mouse)
• Satomi Kagota, Kana Maruyama-Fumoto, Saki Iwata, Miho Shimari, Shiori Koyanagi, Yayoi Shiokawa et al. Perivascular Adipose Tissue-Enhanced Vasodilation in Metabolic Syndrome Rats by Apelin and N-Acetyl–l-Cysteine-Sensitive Factor(s) International Journal of Molecular Sciences 2018-12-28 [PMID: 30597883]
• SS Rao, Y Hu, PL Xie, J Cao, ZX Wang, JH Liu, H Yin, J Huang, YJ Tan, J Luo, MJ Luo, SY Tang, TH Chen, LQ Yuan, EY Liao, R Xu, ZZ Liu, CY Chen, H Xie Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines Bone Res, 2018-03-30;6(0):9. 2018-03-30 [PMID: 29619269] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITLN1
• Uniprot:
  • O88310()