Integrin beta 7 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Integrin beta 7 Antibody [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
  Glu20-His723
  Accession # P26010
• Specificity: Detects human Integrin beta 7 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Integrin beta 7 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta 2, rhIntegrin beta 5, and rhIntegrin beta 6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGB7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 7 Antibody [Biotin]

• Gut homing receptor beta subunit
• integrin beta 7 subunit
• Integrin beta 7
• integrin beta-7
• integrin, beta 7
• ITGB7

Background

Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20-723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa 501-648. Integrin  beta 7 forms a nondisulfide linked heterodimer with  alpha 4 and  alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1, and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human  beta 7 is 87% aa identical to the ECD of mouse  beta 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB7
• Uniprot:
  • P26010()