Integrin alpha 9 Antibody [Unconjugated]

Integrin alpha 9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin alpha 9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 9 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha 9
  Try31-Val979
  Accession # NP_598482
• Specificity: Detects mouse Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human Integrin alpha 9 is observed and less than 5% cross-reactivity with recombinant mouse Integrin alpha 3 and alpha 4 is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGA9

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 9 Antibody [Unconjugated]

• ALPHA-RLC
• Integrin alpha 9
• integrin alpha-9
• Integrin alpha-RLC
• integrin, alpha 9
• ITGA4L
• ITGA9
• RLC
• RLC-alpha

Background

Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 integrin. alpha 9 beta 1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg566-Val567). The ECD of mouse integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human integrin alpha 9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 9 Antibody (AF3827)(19)

Publications using AF3827

• K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30482854] (IHC-Fr, Mouse)
• T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis Front Immunol, 2018;9(0):334. 2018 [PMID: 29559970] (ICC, Mouse)
• F Anjum, NA Agabalyan, HD Sparks, NL Rosin, MS Kallos, J Biernaskie Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure Sci Rep, 2017;7(1):10291. 2017 [PMID: 28860484] (ICC, Human)
• Alexander M Simon Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43 Dev. Biol., 2016;0(0):. 2016 [PMID: 27899284] (IHC, Mouse)
• Integrin Alpha 9 Blockade Suppresses Lymphatic Valve Formation and Promotes Transplant Survival Invest. Ophthalmol. Vis. Sci., 2016;57(14):5935-5939. 2016 [PMID: 27806381] (IHC, Mouse)
• Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Dev Biol, 2016;409(1):218-33. 2016 [PMID: 26542011] (IHC, Mouse)
• Ruppert R, Moser M, Sperandio M, Rognoni E, Orban M, Liu W, Schulz A, Oostendorp R, Massberg S, Fassler R Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells. J Exp Med, 2015;212(9):1415-32. 2015 [PMID: 26282877] (Flow Cytometry, Mouse)
• Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J Structural and functional features of central nervous system lymphatic vessels. Nature, 2015;523(7560):337-41. 2015 [PMID: 26030524] (IHC, Mouse)
• Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel. J Clin Invest, 2014;124(9):3975-86. 2014 [PMID: 25061878] (IHC-Fr, Mouse)
• Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity. J Clin Invest, 2014;124(9):3960-74. 2014 [PMID: 25061877]
• Zheng W, Nurmi H, Appak S, Sabine A, Bovay E, Korhonen E, Orsenigo F, Lohela M, D'Amico G, Holopainen T, Leow C, Dejana E, Petrova T, Augustin H, Alitalo K Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions. Genes Dev, 2014;28(14):1592-603. 2014 [PMID: 25030698] (IHC-Fr, Mouse)
• Truong, Tan, Huang, Eric, Yuen, Don, Chen, Lu Corneal lymphatic valve formation in relation to lymphangiogenesis. Invest Ophthalmol Vis Sci, 2014;55(3):1876-83. 2014 [PMID: 24595382] (IHC, Mouse)
• Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation. J. Cell Biol., 2012;197(6):837-49. 2012 [PMID: 22665518] (IHC, Mouse)
• Sato-Nishiuchi R, Nakano I, Ozawa A, Sato Y, Takeichi M, Kiyozumi D, Yamazaki K, Yasunaga T, Futaki S, Sekiguchi K Polydom/SVEP1 Is a Ligand for Integrin alpha9beta1. J. Biol. Chem., 2012;287(30):25615-30. 2012 [PMID: 22654117] (IHC-Fr, Mouse)
• Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood, 2012;119(23):5429-37. 2012 [PMID: 22553313] (Flow Cytometry, Human)
• Bazigou E, Lyons OT, Smith A, Venn GE, Cope C, Brown NA, Makinen T Genes regulating lymphangiogenesis control venous valve formation and maintenance in mice. J. Clin. Invest., 2011;121(8):2984-92. 2011 [PMID: 21765212] (IHC-Fr, Human)
• Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells. Stem Cells, 2011;29(6):964-71. 2011 [PMID: 21563278] (Flow Cytometry, Mouse)
• Tsai SY, Clavel C, Kim S, Ang YS, Grisanti L, Lee DF, Kelley K, Rendl M Oct4 and klf4 reprogram dermal papilla cells into induced pluripotent stem cells. Stem Cells, 2010;28(2):221-8. 2010 [PMID: 20014278] (IHC, Mouse)
• Murugaiyan G, Mittal A, Weiner HL Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis. J. Immunol., 2008;181(11):7480-8. 2008 [PMID: 19017937] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ITGA9
• Uniprot:
  • NP_598482()