Integrin beta 5 Antibody (2C4) - Azide and BSA Free

Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 expression in transfected 293T cell line by ITGB5 monoclonal antibody (M01), clone 2C4.Lane 1: ITGB5 transfected lysate(88.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Analysis of ITGB5 over-expressed 293 cell line, cotransfected with ITGB5 Validated Chimera RNAi ( Cat # H00003693-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITGB5 monoclonal antibody (M01), clone 2C4 (Cat # H00003693-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Integrin beta 5 Antibody (2C4) [H00003693-M01] - ITGB5 monoclonal antibody (M01), clone 2C4 Analysis of ITGB5 expression in HeLa.

ELISA: Integrin beta 5 Antibody (2C4) [H00003693-M01] - Detection limit for recombinant GST tagged ITGB5 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, KD
• Clone: 2C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Integrin beta 5 Antibody (2C4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITGB5 (NP_002204, 421 a.a. ~ 516 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREA
• Specificity: ITGB5 - integrin, beta 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Integrin beta 5 Antibody (2C4) - Azide and BSA Free

• FLJ26658
• Integrin beta 5
• integrin beta-5
• integrin, beta 5
• ITGB5

Background

Integrins are heterodimers composed of noncovalently associated transmembrane

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 5 Antibody (H00003693-M01)(3)

Publications using H00003693-M01

• D Zang, C Zhang, C Li, Y Fan, Z Li, K Hou, X Che, Y Liu, X Qu LPPR4 promotes peritoneal metastasis via Sp1/integrin &alpha/FAK signaling in gastric cancer Am J Cancer Res, 2020-03-01;10(3):1026-1044. 2020-03-01 [PMID: 32266108]
• Sa S, Wong L, McCloskey KE. Combinatorial fibronectin and laminin signaling promote highly efficient cardiac differentiation of human embryonic stem cells. Biores Open Access 2014-08-01 [PMID: 25126479]
• Ballana E, Pauls E, Clotet B et al. beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication. J Immunol. 2010-11-22 [PMID: 21098231]

Bioinformatics

• Gene Symbol: ITGB5
• Entrez:
  • 3693(Human)
• OMIM:
  • 147561(Human)
• Uniprot:
  • NP_002204()