Integrin beta 3/CD61 Antibody (F11) - BSA Free

Flow Cytometry: Integrin beta 3/CD61 Antibody (F11) [NB100-65387] - Figure A. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD63 and FITC conjugated mouse anti rat CD61. All experiments performed on rat peritoneal macrophages.

Flow Cytometry: Integrin beta 3/CD61 Antibody (F11) [NB100-65387] - Staining of rat peripheral blood platelets with Alexa Fluor® ?647 conjugated Mouse anti Rat CD61.

Flow Cytometry: Integrin beta 3/CD61 Antibody (F11) [NB100-65387] - Figure A. FITC conjugated mouse anti rat CD61 and A647 conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti rat CD61 and A647 conjugated mouse anti rat CD63. All experiments performed on rat peritoneal macrophages.

Product Discontinued

Integrin beta 3/CD61 Antibody (F11) - BSA Free Summary

• Immunogen: Bone cell suspension from SD rats
• Localization: Cell Membrane
• Specificity: NB100-65387 recognises the rat CD61 cell surface antigen, also known as the integrin b3 chain. CD61 is expressed by platelets, megakaryocytes and osteoclasts.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Reacts weakly with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 3/CD61 Antibody (F11) - BSA Free

• CD61 antigen
• CD61
• GP3A
• GP3Aplatelet glycoprotein IIIa
• GPIIIA
• GPIIIaintegrin beta-3
• INGRB3
• Integrin beta 3
• integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• ITG B3
• ITGB3
• Platelet membrane glycoprotein IIIa

Background

The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB3