Home » Integrin beta 3/CD61 » Integrin beta 3/CD61 Antibodies » Integrin beta 3/CD61 Antibody (909114) [Alexa Fluor® 594]

Integrin beta 3/CD61 Antibody (909114) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
909114
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB41182U-100UG
Alexa Fluor 405
FAB41182V-100UG
Alexa Fluor 488
FAB41182G-100UG
Alexa Fluor 647
FAB41182R-100UG
Alexa Fluor 700
FAB41182N-100UG
Alexa Fluor 750
FAB41182S-100UG
Unconjugated
MAB41182
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB41182
Carrier Free
MAB41182
Carrier Free
FAB41182V-100UG
Carrier Free
FAB41182U-100UG
Carrier Free
FAB41182S-100UG
Carrier Free
FAB41182R-100UG
Carrier Free
FAB41182N-100UG
Carrier Free
FAB41182G-100UG
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Integrin beta 3/CD61 Antibody (909114) [Alexa Fluor® 594] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b and Integrin beta 3 linked heterodimer
Leu32-Arg988 (Integrin alpha 2b) & Glu26-Asp717 (Integrin beta 3)
Accession # NP_034705 (Integrin alpha 2b) & O54890 (Integrin beta 3)
Specificity
Detects mouse Integrin  beta 3/CD61 in direct ELISA.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
ITGB3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 3/CD61 Antibody (909114) [Alexa Fluor® 594]

  • CD61 antigen
  • CD61
  • GP3A
  • GP3Aplatelet glycoprotein IIIa
  • GPIIIA
  • GPIIIaintegrin beta-3
  • INGRB3
  • Integrin beta 3
  • integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
  • ITG B3
  • ITGB3
  • Platelet membrane glycoprotein IIIa

Background

Integrin beta 3 (ITGB3) is also known as platelet glycoprotein IIIa (GPIIIa) and CD61. Integrins are integral cell-surface proteins known to participate in both cell adhesion and cell-surface mediated signaling. They are composed of an alpha chain and a beta chain. ITGB3 is an integrin beta chain that forms a heterodimer with alpha-IIb or alpha-V chains. It is involved in platelet aggregation, and serves as an anchor for fibrinogen, allowing platelets to form aggregates or clots. A functional absense of ITGB3 leads to Glanzmann's thrombasthenia, a condition where platelets are activated, but fail to form an aggregate. Alternatively spliced transcripts encoding different proteins have been described, in human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/mL). Detection by DAB.
Western Blot: SP1 Antibody [NB600-232] - A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/mL for WB. Detection by chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
(1 Review)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB41182U
Alexa Fluor 405 FAB41182V
Alexa Fluor 488 FAB41182G
Alexa Fluor 594 FAB41182T
Alexa Fluor 647 FAB41182R
Alexa Fluor 700 FAB41182N
Alexa Fluor 750 FAB41182S
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