Integrin beta 2/CD18 Antibody (MEM48) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, Flow, Func, IHC, IP
• Clone: MEM48
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Integrin beta 2/CD18 Antibody (MEM48) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Leucocytes from an LGL - type leukaemia
• Specificity: Recognizes the CD18 molecule, a 95 kDa integrin molecule (Integrin beta) that forms the common beta chain of the CD11/CD18 heterodimer integrins.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Integrin beta 2/CD18 Antibody (MEM48) [CoraFluor™ 1]

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB2