Integrin beta 2/CD18 Antibody (68-5A5)

Product Discontinued

Integrin beta 2/CD18 Antibody (68-5A5) Summary

• Immunogen: Stimulated human leukocytes
• Localization: Cell Surface
• Specificity: It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers, LFA-1, Mac-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54), ICAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and iC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This MAb blocks these cellular interactions.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Functional Studies: Order Ab without Azide.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin beta 2/CD18 Antibody (68-5A5)

• 959 beta subunit precursor)10
• CD18 antigen
• CD18
• CD18beta 2
• cell surface adhesion glycoprotein (LFA-1
• CR3
• Integrin beta 2
• integrin beta-2
• integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
• integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• ITGB2
• LAD
• LCAMB
• leukocyte cell adhesion molecule CD18
• leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
• LFA-1 beta
• LFA-1
• MAC-1 beta
• MF17
• MFI7
• P150
• p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGB2