Integrin beta 1/CD29 Antibody (4B7R) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Integrin beta 1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
• Clone: 4B7R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 1/CD29 Antibody (4B7R) [Unconjugated] Summary

• Immunogen: Ocular melanoma cell line V+B2
• Specificity: Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody (4B7R) [Unconjugated]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits (1).

1. Wayner, E.A. and W.G. Carter (1987) J. Cell Biol. 105:1873.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (MAB1778)(9)

Publications using MAB1778

• Pei Shen, Lu Chen, Dahe Zhang, Simo Xia, Zhuman Lv, Duohong Zou et al. Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs npj Regenerative Medicine 12/8/2022 [PMID: 36477591]
• Virga J, Szivos L et al. Extracellular matrix differences in glioblastoma patients with different prognoses. Oncol Lett 1/1/2019 [PMID: 30655832]
• Clayton S Lewis, Hala Elnakat Elnakat Thomas, Melissa Orr‐Asman, Lisa C Green, Rachel E Boody, Kateryna Matiash et al. mTOR kinase inhibition reduces tissue factor expression and growth of pancreatic neuroendocrine tumors Journal of Thrombosis and Haemostasis 1/1/2019 [PMID: 30472780]
• Qinghua Ye, Lei Lei, Lingyun Shao, Jing Shi, Jun Jia, Xiaowen Tong MicroRNA-141 inhibits epithelial-mesenchymal transition, and ovarian cancer cell migration and invasion Molecular Medicine Reports 9/13/2017 [PMID: 28901523]
• SIN-HYE PARK, MIN JAE SHIN, DAE WON KIM, JINSEU PARK, SOO YOUNG CHOI, YOUNG-HEE KANG Blockade of monocyte-endothelial trafficking by transduced Tat-superoxide dismutase protein International Journal of Molecular Medicine 2/1/2016 [PMID: 26707483]
• Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen C, Kroll A, Carpenter C, Ramesh M, Qu V, Patel S, Zhu J, Shi W, Hofman F, Chen T, Gao W, Zhang K, Chien S, Zhang L Nanoparticle biointerfacing by platelet membrane cloaking. Nature, 2015-09-16;526(7571):118-21. 2015-09-16 [PMID: 26374997] (Western Blot, Human)
• Levi L, Toyooka T, Patarroyo M, Frisan T Bacterial genotoxins promote inside-out integrin beta1 activation, formation of focal adhesion complexes and cell spreading. PLoS ONE, 2015-04-13;10(4):e0124119. 2015-04-13 [PMID: 25874996] (Flow Cytometry, Human)
• Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease. Infect. Immun., 2006-10-02;74(12):7010-3. 2006-10-02 [PMID: 17015454] (IHC-P, Human)
• Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human)

FAQs for Integrin beta 1/CD29 Antibody (MAB1778). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1