Integrin beta 1/CD29 Antibody (419127) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Integrin beta 1/CD29 was detected in immersion fixed porcine mesenchymal stem cells using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Porcine/Equine Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17783) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

• Reactivity: Hu, Po, Eq
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 419127
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin beta 1/CD29 Antibody (419127) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29
  Gln21-Asp728 (predicted)
  Accession # P05556
• Specificity: Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody (419127) [Unconjugated]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (MAB17783)(2)

Publications using MAB17783

• Kwang-Hwan Choi, Minsu Kim, Ji Won Yoon, Jinsol Jeong, Minkyung Ryu, Cheorun Jo et al. Purification of Pig Muscle Stem Cells Using Magnetic-Activated Cell Sorting (MACS) Based on the Expression of Cluster of Differentiation 29 (CD29) Food Science of Animal Resources 2020-09-01 [PMID: 32968735] (Flow Cytometry, Immunocytochemistry, Porcine)
• Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture. Exp. Cell Res., 2010-11-04;317(3):276-92. 2010-11-04 [PMID: 21056557] (Flow Cytometry, Human)

FAQs for Integrin beta 1/CD29 Antibody (MAB17783). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Uniprot:
  • P05556()