Integrin beta 1/CD29 Antibody (265917) [Phycoerythrin]

B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB2405P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 265917
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Integrin beta 1/CD29 Antibody (265917) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
  Gln21-Asp728
  Accession # P09055
• Specificity: Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 3, rmIntegrin beta 4, or rmIntegrin beta 6 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody (265917) [Phycoerythrin]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (FAB2405P)(7)

Publications using FAB2405P

• Latifa Mohamed Abdelgawad, Manar Mohy Abd El-hamed, Dina Sabry, Marwa Abdelgwad, Department of Medical Laser applications, National Institute of laser enhanced science, Cairo University, Egypt., Department of Medical Laser application, National Institute of laser enhanced science, Cairo University, Egypt et al. Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway Reports of Biochemistry and Molecular Biology 4/1/2021 [PMID: 34277866]
• Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression Biomolecular Concepts 12/23/2020 [PMID: 34233429]
• Latifa Mohamed Abdelgawad, Asmaa Mohamed Abdelaziz, Dina Sabry, Marwa Abdelgwad Influence of photobiomodulation and vitamin D on osteoblastic differentiation of human periodontal ligament stem cells and bone-like tissue formation through enzymatic activity and gene expression Biomolecular Concepts 2020-12-23 [PMID: 34233429] (Flow Cytometry, Human)
• Catalina-Iolanda Marinescu, Mihai Bogdan Preda, Alexandrina Burlacu A procedure for in vitro evaluation of the immunosuppressive effect of mouse mesenchymal stem cells on activated T cell proliferation Stem Cell Research & Therapy 2021-06-05 [PMID: 34090507]
• A Filippi, A Constantin, N Alexandru, G Voicu, CA Constantin, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu Integrins alpha4beta1 and alphaVbeta3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease Cell Transplant, 2020-01-01;29(0):9636897209462. 2020-01-01 [PMID: 32841051] (Flow Cytometry, Mouse, Transgenic Mouse)
• Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression. J Immunol, 2013-12-04;192(1):407-17. 2013-12-04 [PMID: 24307731] (Flow Cytometry, Mouse)
• Chih-Lung Chen, Haosen Zhang, Qing Ye, Wen-Yuan Hsieh, T. Kevin Hitchens, Hsin-Hsin Shen et al. A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging Molecular Imaging and Biology 2011-10-01 [PMID: 20862612] (Flow Cytometry, Rat)

FAQs for Integrin beta 1/CD29 Antibody (FAB2405P). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Uniprot:
  • P09055()