Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free

Immunoprecipitation: Integrin alpha V beta 6 Antibody (AvB6 53a.2) [NBP2-50449] - avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds, and 53a2 shows only the upper band of B6. By 1 minute, most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.

• Reactivity: Hu
• Applications: Flow, Func, ICC/IF, IP, CyTOF-ready
• Clone: AvB6 53a.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free Summary

• Immunogen: Mouse fibroblasts retrovirally infected with cDNA to human Integrin alpha V beta 6 (3T3b6.19). Cells expressed a mouse/human Integrin alpha V beta 6 chimera.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGAV
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Positive control(s): BT20 breast carcinoma, original immunogen. This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin alpha V beta 6 Antibody (AvB6 53a.2) - BSA Free

• CD51
• Integrin alpha V beta 6
• integrin subunit alpha V
• ITGAV
• MSK8
• VNRA
• VTNR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V beta 6 Antibody (NBP2-50449)(1)

Publication using NBP2-50449

• Kim E J, Kwon K A et al. Korean Red Ginseng extract reduces hypoxia-induced epithelial-mesenchymal transition by repressing NF-kappa B and ERK1/2 pathways in colon cancer. J Ginseng Res 2018-01-07 [PMID: 29983610] (WB, Human)

FAQs for Integrin alpha V beta 6 Antibody (NBP2-50449). (Showing 1 - 1 of 1 FAQ).

1. Is your Integrin alpha V beta 6 Antibody (AvB6 53a.2) a mixed clone?
   • Yes, this monoclonal antibody contain two clones: clones AvB6 and 53a.2 which are against Integrin alpha V and Integrin beta 6, respectively.  Clone 53a2 is against human alpha v beta 6 (PMID: 18451156 ). Clone AvB6 was produced using immunogen "Mouse fibroblasts retrovirally infected with cDNA to human beta6 (3T3b6.19). The cells expressed a mouse/human avb6 chimera  i.e. Integrin alpha V is from mouse, whereas beta 6 is from human.

Bioinformatics

• Gene Symbol: ITGAV