Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free

Surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (concentration in sample 1 ug/ml) GAM APC.

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free [NB500-309] - Surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free [NB500-309] - Surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

• Reactivity: Hu
• Applications: Flow, IP
• Clone: MEM-83
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free Summary

• Description: Novus Biologicals Mouse Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free (NB500-309) is a monoclonal antibody validated for use in Flow and IP. Anti-Integrin alpha L/CD11a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone MEM-83 was used by HLDA to establish CD designation.
• Immunogen: Human peripheral blood lymphocytes.
• Specificity: The antibody MEM-83 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV; WS Code N 211
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: Functional Application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Integrin alpha L/CD11a Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha L/CD11a Antibody (MEM-83) - BSA Free

• CD11 antigen-like family member A
• CD11a antigen
• CD11a
• CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
• Integrin alpha L
• integrin alpha-L
• integrin gene promoter
• integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
• ITGAL
• Leukocyte adhesion glycoprotein LFA-1 alpha chain
• Leukocyte function-associated molecule 1 alpha chain
• LFA-1 alpha
• LFA-1
• LFA1A
• LFA-1A
• Ly-15
• Ly-21
• lymphocyte function-associated antigen 1

Background

CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha L/CD11a Antibody (NB500-309)(1)

Publication using NB500-309

• Liu X, Gibbons RM, Harrington SM et al. Endogenous tumor-reactive CD8+ T cells are differentiated effector cells expressing high levels of CD11a and PD-1 but are unable to control tumor growth. Oncoimmunology 2013-06-01 [PMID: 23894697] (FLOW, Human)

Bioinformatics

• Gene Symbol: ITGAL
• Entrez:
  • 3683(Human)