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Integrin alpha L/CD11a Antibody (CRIS-3)

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Flow Cytometry: Integrin alpha L/CD11a Antibody (CRIS-3) [NBP2-32923] - Flow Cytometric staining of human PBMCs. Integrin alpha L/CD11a Antibody (CRIS-3).
Flow Cytometry: Integrin alpha L/CD11a Antibody (CRIS-3) [NBP2-32923] - Flow Cytometric staining of human PBMCs. Integrin alpha L/CD11a Antibody (CRIS-3).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, PAGE
Clone
CRIS-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34599AF350
Alexa Fluor 405
NBP2-34599AF405
Alexa Fluor 488
NBP2-34599AF488
Alexa Fluor 532
NBP2-34599AF532
Alexa Fluor 594
NBP2-34599AF594
Alexa Fluor 647
NBP2-34599AF647
Alexa Fluor 700
NBP2-34599AF700
Alexa Fluor 750
NBP2-34599AF750
Allophycocyanin
NBP2-34599APC
Allophycocyanin/Cy7
NBP2-34599APCCY7
Biotin
NBP2-34599B
DyLight 350
NBP2-34599UV
DyLight 405
NBP2-34599V
DyLight 488
NBP2-34599G
DyLight 550
NBP2-34599R
DyLight 594
NBP2-34599DL594
DyLight 650
NBP2-34599C
DyLight 680
NBP2-34599FR
DyLight 755
NBP2-34599IR
FITC
NBP2-34599F
HRP
NBP2-34599H
Janelia Fluor 549
NBP2-34599JF549
Janelia Fluor 646
NBP2-34599JF646
PE
NBP2-34599PE
PE/Atto594
NBP2-34599PEATT594
PE/Cy5.5
NBP2-34599PECY55
PE/Cy7
NBP2-34599PECY7
PerCP
NBP2-34599PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-34599-0.1 mg
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Integrin alpha L/CD11a Antibody (CRIS-3) Summary

Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop II; Code N202). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ITGAL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • SDS-Page
Application Notes
ELISA (For coating, order NBP2-34599 (BSA free version of NBP2-32923))
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Integrin alpha L/CD11a Antibody (CRIS-3)

  • CD11 antigen-like family member A
  • CD11a antigen
  • CD11a
  • CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
  • Integrin alpha L
  • integrin alpha-L
  • integrin gene promoter
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
  • ITGAL
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • Leukocyte function-associated molecule 1 alpha chain
  • LFA-1 alpha
  • LFA-1
  • LFA1A
  • LFA-1A
  • Ly-15
  • Ly-21
  • lymphocyte function-associated antigen 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: CD11b Antibody [NB110-89474] - A surface stain was performed on Raw264.7 cells with DyLight 550-conjugated [NB110-89474R] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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TNF-alpha
[Unconjugated]
210-TA
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Integrin alpha
L/CD11a Antibody (CRI ...
NBP2-32923
Integrin alpha L/CD11a Antibody (CRIS-3)
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Applications: Flow, ICC/IF, IHC, IHC-Fr, PAGE

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34599AF350
Alexa Fluor 405 NBP2-34599AF405
Alexa Fluor 488 NBP2-34599AF488
Alexa Fluor 532 NBP2-34599AF532
Alexa Fluor 594 NBP2-34599AF594
Alexa Fluor 647 NBP2-34599AF647
Alexa Fluor 700 NBP2-34599AF700
Alexa Fluor 750 NBP2-34599AF750
Allophycocyanin NBP2-34599APC
Allophycocyanin/Cy7 NBP2-34599APCCY7
Biotin NBP2-34599B
DyLight 350 NBP2-34599UV
DyLight 405 NBP2-34599V
DyLight 488 NBP2-34599G
DyLight 550 NBP2-34599R
DyLight 594 NBP2-34599DL594
DyLight 650 NBP2-34599C
DyLight 680 NBP2-34599FR
DyLight 755 NBP2-34599IR
FITC NBP2-34599F
HRP NBP2-34599H
Janelia Fluor 549 NBP2-34599JF549
Janelia Fluor 646 NBP2-34599JF646
PE NBP2-34599PE
PE/Atto594 NBP2-34599PEATT594
PE/Cy5.5 NBP2-34599PECY55
PE/Cy7 NBP2-34599PECY7
PerCP NBP2-34599PCP
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