Integrin alpha 4/CD49d Antibody (7.2R) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d PE-conjugated Monoclonal Antibody (Catalog # FAB1354P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 7.2R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Integrin alpha 4/CD49d Antibody (7.2R) [Phycoerythrin] Summary

• Immunogen: DX-3 human melanoma cell line
• Specificity: Detects the human Integrin alpha 4/CD49d subunit in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 4/CD49d Antibody (7.2R) [Phycoerythrin]

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

The Integrin  alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 (VLA4) and alpha 4 beta 7 are both receptors for fibronectin and VCAM. Integrin alpha 4 beta 7 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1). On the cell surface, VLA4 will participate in homotypic binding between endothelial cells and leukocytes. The extracellular domains of human and mouse Integrin alpha 4 share 85% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4/CD49d Antibody (FAB1354P)(2)

Publications using FAB1354P

• Bipasha Mukherjee-Clavin, Ruifa Mi, Barbara Kern, In Young Choi, Hotae Lim, Yohan Oh et al. Comparison of three congruent patient-specific cell types for the modelling of a human genetic Schwann-cell disorder Nature Biomedical Engineering 2019-04-08 [PMID: 30962586]
• Bipasha Mukherjee-Clavin, Ruifa Mi, Barbara Kern, In Young Choi, Hotae Lim, Yohan Oh et al. Comparison of three congruent patient-specific cell types for the modelling of a human genetic Schwann-cell disorder Nature Biomedical Engineering 2019-04-08 [PMID: 30962586] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGA4