Integrin alpha 4/CD49d Antibody (2C11)

Western Blot: Integrin alpha 4/CD49d Antibody (2C11) [H00003676-M01] - ITGA4 monoclonal antibody (M01), clone 2C11 Analysis of ITGA4 expression in Jurkat.

ELISA: Integrin alpha 4/CD49d Antibody (2C11) [H00003676-M01] - Detection limit for recombinant GST tagged ITGA4 is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Integrin alpha 4/CD49d Antibody (2C11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITGA4 (NP_000876, 98 a.a. ~ 207 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAG
• Specificity: ITGA4 - integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27364558). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Integrin alpha 4/CD49d Antibody (2C11)

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4/CD49d Antibody (H00003676-M01)(2)

Publications using H00003676-M01

• Beauvais DM, Jung O, Yang Y et al. Syndecan-1 (CD138) activates IGF1R and suppresses apoptosis in multiple myeloma: blockade by synstatinIGF1R inhibits tumor growth in vivo. Cancer Res. 2016-06-30 [PMID: 27364558] (WB, Mouse)
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: ITGA4
• Entrez:
  • 3676(Human)
• OMIM:
  • 192975(Human)
• Uniprot:
  • NP_000876()