Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu143) [Unconjugated]

Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta 7 (Research Grade Abrilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10548) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: Hu143
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu143) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human Integrin alpha4 beta7
• Specificity: Detects Human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Abrilumab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu143) [Unconjugated]

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49d/beta7
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4 beta 7
• integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• LPAM1
• LPAM-1
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha

Background

Abrilumab is an anti-human alpha 4 beta 7 integrin heterodimer monoclonal antibody designed for the treatment of inflammatory bowel disease, ulcerative colitis, and Crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.