Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu117) [Unconjugated]

Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta 7/LPAM-1 (Research Grade Vedolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10078) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: Hu117
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu117) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: Human Integrin alpha 4 beta 7/LPAM‑1
• Specificity: Detects human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Vedolizumab. This product is for research use only.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: ITGA4
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 4 beta 7/LPAM-1 Antibody (Hu117) [Unconjugated]

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49d/beta7
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4 beta 7
• integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• LPAM1
• LPAM-1
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha

Background

Vedolizumab is a biosimilar that binds and blocks binds to integrin alpha 4 beta 7. This product can be used in flow cytometry or various assay formats to block or measure integrin alpha 4 beta 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4 beta 7/LPAM-1 Antibody (MAB10078)(3)

Publications using MAB10078

• Mann, BT;Sanz, M;Clohosey, M;Langlands, K;Chitrakar, A;Moreno, C;Vitalle, J;Iannone, MA;Ruiz-Mateos, E;Deleage, C;Siegel, M;Soriano-Sarabia, N; Dual role of circulating and mucosal V?1 T cells in the control of and contribution to persistent HIV-1 infection Research square 2024-08-02 [PMID: 39149467] (Flow Cytometry, Human)
• J Chepngeno, JO Amimo, H Michael, K Jung, S Raev, MV Lee, D Damtie, AO Mainga, AN Vlasova, LJ Saif Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A Viruses, 2022-10-26;14(11):. 2022-10-26 [PMID: 36366453] (Flow Cytometry, Porcine)
• Brecht Creyns, Inge Jacobs, Bram Verstockt, Jonathan Cremer, Vera Ballet, Roselien Vandecasteele et al. Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium Frontiers in Immunology 8/27/2020 [PMID: 32983101]

Bioinformatics

• Gene Symbol: ITGA4