Integrin alpha 3/CD49c Antibody (CL6937) - BSA Free

Western Blot: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Analysis in human cell line U-87 MG.

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Analysis in human lung and liver tissues. Corresponding ITGA3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Staining of human lung shows strong membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Staining of human colon shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Integrin alpha 3/CD49c Antibody (CL6937) [NBP2-76515] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.

• Reactivity: Hu
• Applications: WB, IHC, KD
• Clone: CL6937
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Integrin alpha 3/CD49c Antibody (CL6937) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P26006, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84)% and Rat (82)%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, containing 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Integrin alpha 3/CD49c Antibody (CL6937) - BSA Free

• antigen identified by monoclonal J143
• CD49 antigen-like family member C
• CD49c antigen
• CD49c
• FLJ34631
• FLJ34704
• FRP-2
• Galactoprotein B3
• GAP-B3
• GAPB3CD49C
• Integrin alpha 3
• integrin alpha-3
• integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• ITGA3
• MSK18
• VCA-2
• very late activation protein 3 receptor, alpha-3 subunit
• VL3A
• VLA-3 subunit alpha
• VLA3a

Background

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 (VLA3), very common antigen 2 (VCA2), extracellular matrix receptor 1 (ECMR1), and galactoprotein b3 (GAPB3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA3