Integrin alpha 2b/CD41 Antibody (MWReg30) - BSA Free

Flow Cytometry: Integrin alpha 2b/CD41 Antibody (MWReg30) [NBP1-43415] - Analysis using the Biotin conjugate of NBP1-43415. Staining of mouse platelets with Anti-Mouse/Rat CD61 (Integrin beta 3) FITC and 0.06 ug of Rat IgG1 K Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse CD41 Biotin (right) followed by Streptavidin PE.

Flow Cytometry: Integrin alpha 2b/CD41 Antibody (MWReg30) [NBP1-43415] - Staining of mouse platelets with 0.125 ug of Rat IgG1 Isotype Control Purified (left) or 0.125 ug of Anti-Mouse CD41 Purified (right) followed by Anti-Rat IgG PE .

• Reactivity: Mu
• Applications: ELISA, Flow, IHC, IP
• Clone: MWReg30
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Integrin alpha 2b/CD41 Antibody (MWReg30) - BSA Free Summary

• Description: Novus Biologicals Rat Integrin alpha 2b/CD41 Antibody (MWReg30) - BSA Free (NBP1-43415) is a monoclonal antibody validated for use in IHC, ELISA, Flow and IP. Anti-Integrin alpha 2b/CD41 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CD41.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA2B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
• Application Notes: This MWReg30 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 22262462).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 2b/CD41 Antibody (MWReg30) - BSA Free

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

The MWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alphaIIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin betaIII) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin. Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 2b/CD41 Antibody (NBP1-43415)(3)

Publications using NBP1-43415

• Leberzammer J, Agten SM, Blanchet X et al. Targeting platelet-derived CXCL12 impedes arterial thrombosis Blood 2022-03-21 [PMID: 35313337] (FLOW, Mouse)
• Iso-o N, Komatsuya K, Tokumasu F Et al. Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins Frontiers in Cell and Developmental Biology 2021-11-11 [PMID: 34858976] (S-ELISA, Mouse)
• Saitoh N, Sakamoto C, Hagiwara M et al. The distribution of phosphorylated SR proteins and alternative splicing are regulated by RANBP2. Mol Biol Cell. 2012-03-01 [PMID: 22262462] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ITGA2B