Integrin alpha 2b/CD41 Antibody (386629) [Unconjugated]

Integrin a2b/CD41 was detected in immersion fixed mouse bone marrow cells using Rat Anti-Mouse Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB41181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 386629
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 2b/CD41 Antibody (386629) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b/CD41
  Leu32-Arg988
  Accession # NP_034705
• Specificity: Detects mouse Integrin alpha 2b/CD41 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA2B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2b/CD41 Antibody (386629) [Unconjugated]

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

Integrin alpha 2b, (ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. ITGA2b associates with integrin beta 3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. ITGA2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 2b/CD41 Antibody (MAB41181)(1)

Publication using MAB41181

• T Liu, NA Barrett, Y Kanaoka, K Buchheit, TM Laidlaw, D Garofalo, J Lai, HR Katz, C Feng, JA Boyce Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism Mucosal Immunol, 2019-01-21;0(0):. 2019-01-21 [PMID: 30664709] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ITGA2B
• Uniprot:
  • NP_034705()