Insulin Receptor beta Antibody - BSA Free

Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Insulin Receptor, b subunit (~97kDa)

Immunoprecipitation: Insulin Receptor beta Antibody [NB120-10991] - Immunoprecipitation 10 ug of this antibody immunoprecipitated a-Insulin Receptor, Beta subunit from 500 micrograms of Hek293 cell lysate using protein A agarose beads. Arrow indicates a-Insulin Receptor, Beta subunit (97 kDa).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Insulin Receptor beta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Insulin Receptor beta Antibody - BSA Free (NB120-10991) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Protein corresponding to the last 100 C-terminal amino acid residues of the human insulin receptor beta chain. NCBI accession no. NP_000199.
• Epitope: C-terminus
• Localization: Type I membrane protein.
• Specificity: Insulin Receptor beta
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INSR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:100 - 1:500
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Chimpanzee.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1 M Tris-Glycine (pH 7.4), 0.15 M NaCl, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Insulin Receptor beta Antibody - BSA Free

• CD220 antigen
• CD220
• EC 2.7.10
• EC 2.7.10.1
• HHF5
• insulin receptor
• IR

Background

The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INSR
• Entrez:
  • 3643(Human)