Inhibin alpha Antibody (4A2)

Immunocytochemistry/Immunofluorescence: Inhibin alpha Antibody (4A2) [NB110-89459] - Analysis of Hela cells using INHA mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: Inhibin alpha Antibody (4A2) [NB110-89459] - Analysis of human lymphoid (A), ovary tumor (B) and testicle tumor (C) tissues using INHA mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Inhibin alpha Antibody (4A2) Summary

• Immunogen: Purified recombinant fragment of human INHA expressed in E. Coli.
• Specificity: INHA (4A2F7=4A2F2)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INHA
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: Western Blot reported in scientific literature (PMID: 23564191).
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Ascites

Alternate Names for Inhibin alpha Antibody (4A2)

• A-inhibin subunit
• INHA
• Inhibin A
• inhibin alpha chain
• inhibin, alpha

Background

INHA(A-inhibin subunit precursor, inhibin alpha subunit ),also called inhibin(alpha) ,which is located on chromosome 2q33-q36. Inhibin is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises of two subunits,Inhibin A and B. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa cell tumors and can therefore be used as a marker for primary as well as recurrent disease. In addition to their role in endocrine feedback in the reproductive sytem, inhibins subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal,bone marrow, placenta, and most notably anterior pituitary. Inhibin alpha subunit gene expression is down regulated in human prostate cancer, suggesting a tumor suppressive role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Inhibin alpha Antibody (NB110-89459)(1)

Publication using NB110-89459

• Chauhan SK, Singh VV, Rai R et al. Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation. J Clin Immunol. 2013-07-01 [PMID: 23564191] (WB)
  
  Details:
  PBLs (WB) or B-cells (Flow intracellular) from SLE patients or controls: 1. TLR7 mAb clone IMG4G6 (IMG-6675A): WB (Figs 2a, 2c).

FAQs for Inhibin alpha Antibody (NB110-89459). (Showing 1 - 3 of 3 FAQ).

1. What is the typical concentration of NB110-89459? Why is it provided in ml not in mg? Are there any proteins in the buffer?
   • Inhibin alpha antibody (4A2) (NB110-89459) is provided at a concentration of 1 mg/ml and it contains PBS and 0.03% sodium azide. It is Protein A column purified antibody and does not contain any other proteins.
2. It looks like the Activin A monoclonal comes in PBS with 5% Trehalose and its conc. after re-constitution with 1 mL of water is 0.5 mg/mL. Is that right? Does it come in higher concentrations?
   • Activin A antibody (9H16) (NBP2-14829) antibody is supplied as 500ug in lyophilized form and you can reconstitute it in 1ml of PBS to achieve 0.5mg/ml concentration. However, the good news is that if you wish to achieve higher concentration, you may reconstitute it in lesser amount of PBS. Suppose, if you reconstitute 500ug of lyophilized antibody in 500ul of PBS, you will get a final concentration of 1mg/ml. And if you reconstitute 500ug of lyophilized antibody in 250ul of PBS, you will get a final concentration of 2mg/ml.
3. Could you please inform me about the antibody concentration in cat. no NB110-89459, lot 070312 which I received today? I'm afraid I cannot find it on the vial label where it is supposed to be found according to the data sheet.
   • The concentration of this antibody is 1.0mg/mL

Bioinformatics

• Gene Symbol: INHA
• Entrez:
  • 3623(Human)
• Uniprot:
  • P05111()