Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric)

ELISA: Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) [NBP2-62765] - Samples were spiked with high concentrations of Human Indoleamine 2,3-dioxygenase/IDO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) [NBP2-62765] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human IDO in samples. No significant cross-reactivity or interference between Human IDO and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6.04%
• Intra-Assay: CV% < 4.98%
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IDO1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric)

• 3dioxygenase
• EC 1.13.11.52
• IDO
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Indoleamine 2,3-dioxygenase/IDO ELISA Kit (NBP2-62765)(6)

Publications using NBP2-62765

• Cai X, Gui RY, Wu J et Al. Decreased Expression of IL-35 and Its Receptor Contributes to Impaired Megakaryopoiesis in the Pathogenesis of Immune Thrombocytopenia Adv Sci (Weinh) 2024-01-15 [PMID: 38225757] (ELISA)
• Xia L, Zheng Z, Liu JY Et al. Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition Cancer immunology research 2021-06-01 [PMID: 33875483] (ELISA)
• Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis Jul 13 1905 (ELISA)
• Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis 1905-07-13 (ELISA)
• Zhang X, Cai Y, Su X et al. Untargeted metabolomics identified kynurenine as a predictive prognostic biomarker in acute myocardial infarction Frontiers in immunology 2022-11-02 [PMID: 36405744]
• Mahapatra S, Sharma MVR, Brownson B et al. Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine signaling Molecular biology of the cell 2022-06-02 [PMID: 35653297] (ELISA)

Bioinformatics

• Gene Symbol: IDO1