Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric)

ELISA: Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) [NBP2-62765] - Samples were spiked with high concentrations of Human Indoleamine 2,3-dioxygenase/IDO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) [NBP2-62765] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human IDO in samples. No significant cross-reactivity or interference between Human IDO and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6.04%
• Intra-Assay: CV% < 4.98%
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IDO1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Indoleamine 2,3-dioxygenase/IDO ELISA Kit (Colorimetric)

• 3dioxygenase
• EC 1.13.11.52
• IDO
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Indoleamine 2,3-dioxygenase/IDO ELISA Kit (NBP2-62765)(6)

Publications using NBP2-62765

• Cai X, Gui RY, Wu J et Al. Decreased Expression of IL-35 and Its Receptor Contributes to Impaired Megakaryopoiesis in the Pathogenesis of Immune Thrombocytopenia Adv Sci (Weinh) 2024-01-15 [PMID: 38225757] (ELISA)
• Xia L, Zheng Z, Liu JY Et al. Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition Cancer immunology research 2021-06-01 [PMID: 33875483] (ELISA)
• Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis Jul 13 1905 (ELISA)
• Mahapatra S Secretion of Cardioprotective Factors from Cardiac Inducing Colonies (Cics) Derived from Germline Pluripotent Stem Cells (Hgpscs) to Combat Myocardial Fibrosis Thesis 1905-07-13 (ELISA)
• Zhang X, Cai Y, Su X et al. Untargeted metabolomics identified kynurenine as a predictive prognostic biomarker in acute myocardial infarction Frontiers in immunology 2022-11-02 [PMID: 36405744]
• Mahapatra S, Sharma MVR, Brownson B et al. Cardiac inducing colonies halt fibroblast activation and induce cardiac/endothelial cells to move and expand via paracrine signaling Molecular biology of the cell 2022-06-02 [PMID: 35653297] (ELISA)

FAQs for Indoleamine 2,3-dioxygenase/IDO ELISA Kit (NBP2-62765). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IDO1