Indoleamine 2,3-dioxygenase/IDO Antibody

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NB100-2459] - (0.5ug/ml) staining of Human Lymph Node (A), Spleen (B) and Placenta (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NB100-2459] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NB100-2459] - (6ug/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Indoleamine 2,3-dioxygenase/IDO Antibody Summary

• Immunogen: Peptide with sequence CVLANWKKKDPNK corresponding to internal region according to NP_002155.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IDO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: Western blot: Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1).Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
  IHC-P: Human colon shows textured staining in the cytoplasm of absorptive cells. Data obtained from a previous batch (different goat).
• Control:

  Indoleamine 2,3-dioxygenase/IDO Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody

• 3dioxygenase
• EC 1.13.11.52
• IDO
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Indoleamine 2,3-dioxygenase/IDO Antibody (NB100-2459)(1)

Publication using NB100-2459

• Munn DH, Sharma MD, Baban B et al. GCN2 kinase in T cells mediates proliferative arrest and anergy induction in response to indoleamine 2,3-dioxygenase. Immunity 2005-05-01 [PMID: 15894280]

FAQs for Indoleamine 2,3-dioxygenase/IDO Antibody (NB100-2459). (Showing 1 - 1 of 1 FAQ).

1. What is the volume of this product?
   • This antibody is 0.1mg at a listed concentration of 0.5 mg/ml. This corresponds to a volume of 200 uL.

Bioinformatics

• Gene Symbol: IDO1
• Entrez:
  • 3620(Human)
• Uniprot:
  • NP_002155.1(Human)