Home » Indoleamine 2,3-dioxygenase/IDO » Indoleamine 2,3-dioxygenase/IDO Antibodies » Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Phycoerythrin]

Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Phycoerythrin]

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Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec‑3/CD33 APC‑conjugated Monoclonal Antibody (Catalog # FAB1137A) and either (A) Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO PE‑conjugated Monoclonal Antibody (Catalog # IC6030P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
700838
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

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Alexa Fluor 350
IC6030U-100UG
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IC6030V-100UG
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IC6030R-100UG
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IC6030A
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IC6030C
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MAB6030
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MAB6030
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MAB6030-SP
Carrier Free
IC6030R-100UG
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Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
Ala2-Gly403
Accession # P14902
Specificity
Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDO1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC6030P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Phycoerythrin]

  • 3dioxygenase
  • EC 1.13.11.52
  • IDO
  • IDO1
  • IDOIDO-1
  • INDO
  • INDOindole 2,3-dioxygenase
  • Indoleamine 2
  • indoleamine 2,3-dioxygenase 1
  • Indoleamine 2,3-dioxygenase
  • indoleamine-pyrrole 2,3 dioxygenase
  • Indoleamine-pyrrole 2,3-dioxygenase

Background

Indoleamine 2,3-dioxygenase (also known as IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N‑formyl‑kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN‑ beta, and IL-10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
  1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
  2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
  3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
  4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
  5. Curti, A. et al. (2009) Blood 113:2394.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Indoleamine
2,3-dioxygenase/IDO A ...
IC6030P
Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Phycoerythrin]
Species: Hu
Applications: Flow

Publications for Indoleamine 2,3-dioxygenase/IDO Antibody (IC6030P)(3)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 2 applications: Flow Cytometry, ICC.


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(1)
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(2)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC6030U
Alexa Fluor 405 IC6030V
Alexa Fluor 488 IC6030G
Alexa Fluor 594 IC6030T
Alexa Fluor 647 IC6030R
Alexa Fluor 750 IC6030S
Allophycocyanin IC6030A
PerCP (Peridinin-chlorophyll Protein Complex) IC6030C
Phycoerythrin IC6030P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol IDO1
Uniprot