Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Alexa Fluor® 488]

Human Monocytes were selected from PBMC using MagCellect Human CD14+ Cell Isolation Kit (MAGH105) and cultured overnight with Recombinant Human MCSF (50 ng/mL; 216-MC), Recombinant Human IFN gamma (50 ng/mL; 285-IF) and 50 ng/mL LPS and stained with (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6030G) or (B) Mouse IgG1 isotype control antibody (IC002G) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6030G) or (B) Mouse IgG1Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 700838
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
  Ala2-Gly403
  Accession # P14902
• Specificity: Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDO1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody (700838) [Alexa Fluor® 488]

• 3dioxygenase
• EC 1.13.11.52
• IDO
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

Indoleamine 2,3-dioxygenase (also known as IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN‑ gamma , IFN-alpha , IFN-beta , and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).

1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
5. Curti, A. et al. (2009) Blood 113:2394.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Indoleamine 2,3-dioxygenase/IDO Antibody (IC6030G)(7)

Publications using IC6030G

• G Gerlini, P Di Gennaro, N Pimpinelli, S Sestini, L Borgognoni Tolerogenic IDO1+CD83- Langerhans Cells in Sentinel Lymph Nodes of Patients with Melanoma International Journal of Molecular Sciences, 2022-03-22;23(7):. 2022-03-22 [PMID: 35408802] (Flow Cytometry, Human)
• HL Huang, HL Yang, ZZ Lai, SL Yang, MQ Li, DJ Li Decidual IDO+ macrophage promotes the proliferation and restricts the apoptosis of trophoblasts Journal of reproductive immunology, 2021-08-23;148(0):103364. 2021-08-23 [PMID: 34482001] (Flow Cytometry, Human)
• SP Sittig, JJP van Beek, G Flórez-Gra, J Weiden, SI Buschow, MC van der Ne, R van Sloote, MM Verbeek, PBH Geurtz, J Textor, CG Figdor, IJM de Vries, G Schreibelt Human type 1 and type 2 conventional dendritic cells express indoleamine 2,3-dioxygenase 1 with functional effects on T cell priming European Journal of Immunology, 2021-03-22;0(0):. 2021-03-22 [PMID: 33675038] (Flow Cytometry, Human)
• Sabrina Sapski, Nadine Beha, Roland E. Kontermann, Dafne Müller Influence of antigen density and immunosuppressive factors on tumor-targeted costimulation with antibody-fusion proteins and bispecific antibody-mediated T cell response Cancer Immunology, Immunotherapy 2020-11-01 [PMID: 32504247]
• Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe J, Harter G, Gruner B, Fabricius D, Lotfi R, Schwarz K, Schutz C, Honig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdorfer B CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. J Immunol, 2015-03-16;194(8):3768-77. 2015-03-16 [PMID: 25780036] (Flow Cytometry, Human)
• Love A, Schwartz I, Petzke M Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential. J Leukoc Biol, 2014-11-24;97(2):379-90. 2014-11-24 [PMID: 25420916] (Flow Cytometry, Human)
• Barbeau , Dominiqu, La , Kiet Tra, Kim , Duk Soo, Kerpedjieva , Svetosla, Shurin , Galina V, Tamama , Kenichi Early growth response-2 signaling mediates immunomodulatory effects of human multipotential stromal cells. Stem Cells Dev, 2013-10-05;23(2):155-66. 2013-10-05 [PMID: 24007274] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IDO1
• Uniprot:
  • P14902()