IMPA1 Antibody (984604) [Unconjugated]

IMPA1/IMP1 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IMPA1/IMP1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human IMPA1/IMP1 Monoclonal Antibody (Catalog # MAB9890) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 984604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IMPA1 Antibody (984604) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human IMPA/IMP1 Peptide
  Accession # P29218
• Specificity: Detects human IMPA1/IMP1 Peptide in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IMPA1 Antibody (984604) [Unconjugated]

• EC 3.1.3.25
• IMP 1
• IMP
• IMP1
• IMPA
• IMPA1
• IMPase 1
• inositol monophosphatase 1
• inositol(myo)-1(or 4)-monophosphatase 1
• Inositol-1(or 4)-monophosphatase 1
• Lithium-sensitive myo-inositol monophosphatase A1
• MRT59
• Myo-Inositol Monophosphatase 1

Background

IMPA-1/inositol(myo)-1(or 4)-monophosphatase 1 is a ubiquitous enzyme with broad specificity, which is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. IMPA-1 dephosphorylates myo-inositol monophosphatate to modulate intracellular signal transduction. IMPA-1 forms homodimers, and is abundantly expressed in brain. The magnesium-dependent phosphatase activity is inhibited by lithium, thereby depressing myo-inositol production, which may explain the anti-depressive and anti-manic effects of lithium therapy in bipolar disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P29218()