ILT2/CD85j/LILRB1 Antibodies

2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND₅₀ is typically 0.025-0.15 µg/mL." class="big_lightbox"> 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND₅₀ is typically 0.025-0.15 µg/mL." alt="Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by acid phosphatase activity. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20172). The ND₅₀ is typically 0.025-0.15 µg/mL." class="big_thumb" />

MAB002, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood monocytes were stained with Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20171, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LILRB1/CD85j/ILT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2017). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_lightbox"> 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LILRB1/CD85j/ILT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2017). The ND₅₀ is typically 0.3-1.0 µg/mL." alt="Recombinant Human LILRB1/CD85j/ILT2j Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LILRB1/CD85j/ILT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2017). The ND₅₀ is typically 0.3-1.0 µg/mL." class="big_thumb" />

HAF018). A specific band was detected for LILRB1/CD85j/ILT2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for LILRB1/CD85j/ILT2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20173) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LILRB1/CD85j/ILT2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by CalceinAM. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20174). The ND₅₀ is typically 0.05-0.4 µg/mL." class="big_lightbox"> 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by CalceinAM. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20174). The ND₅₀ is typically 0.05-0.4 µg/mL." alt="Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog # 2017-T2), immobilized onto a microplate, supports the adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cell line in a dose-dependent manner (orange line), as measured by CalceinAM. Adhesion elicited by Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human LILRB1/CD85j/ILT2 Monoclonal Antibody (Catalog # MAB20174). The ND₅₀ is typically 0.05-0.4 µg/mL." class="big_thumb" />