ILT11/LILRA5 Antibody (711828) [Unconjugated]

Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA5/CD85f Mono­clonal Antibody (Catalog # MAB6754, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

LILRA5/CD85f was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB6754) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 711828
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ILT11/LILRA5 Antibody (711828) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f
  Gly42-Arg268
  Accession # A6NI73
• Specificity: Detects human LILRA5/CD85f in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LILRA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT11/LILRA5 Antibody (711828) [Unconjugated]

• CD85 antigen-like family member F
• CD85
• CD85f antigen
• CD85f
• ILT11
• ILT-11
• ILT11CD85f
• Immunoglobulin-like transcript 11
• leukocyte Ig-like receptor 9
• Leukocyte immunoglobulin-like receptor 9
• leukocyte immunoglobulin-like receptor subfamily A member 5 soluble
• leukocyte immunoglobulin-like receptor subfamily A member 5
• leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRA5
• LILRB7
• LIR9
• LIR-9
• LIR9immunoglobulin-like transcript 11 protein
• member 7

Background

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors.  It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-a and IL-1b.  Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein.  It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail.  ILT11 has three potential splice forms.  One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299.  The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51.  Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LILRA5
• Uniprot:
  • A6NI73(Human)