ILT11/LILRA5 Antibody (711828) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3832C) and either (A) Mouse Anti-Human ILT11/LILRA5 PE-conjugated Monoclonal Antibody (Catalog # FAB6754P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 711828
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ILT11/LILRA5 Antibody (711828) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ILT11/LILRA5
  Gly42-Arg268
  Accession # A6NI73
• Specificity: Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LILRA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT11/LILRA5 Antibody (711828) [Phycoerythrin]

• CD85 antigen-like family member F
• CD85
• CD85f antigen
• CD85f
• ILT11
• ILT-11
• ILT11CD85f
• Immunoglobulin-like transcript 11
• leukocyte Ig-like receptor 9
• Leukocyte immunoglobulin-like receptor 9
• leukocyte immunoglobulin-like receptor subfamily A member 5 soluble
• leukocyte immunoglobulin-like receptor subfamily A member 5
• leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRA5
• LILRB7
• LIR9
• LIR-9
• LIR9immunoglobulin-like transcript 11 protein
• member 7

Background

ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors.  It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-a and IL-1b.  Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein.  It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail.  ILT11 has three potential splice forms.  One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299.  The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51.  Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILT11/LILRA5 Antibody (FAB6754P)(1)

Publication using FAB6754P

• Hong Yu, Hui Liu, Yang Zhao, Huaquan Wang, Chunyan Liu, Weiwei Qi et al. Upregulated expression of leukocyte immunoglobulin‑like receptor A3 in patients with severe aplastic anemia Experimental and Therapeutic Medicine 2021-02-11 [PMID: 33732319]

Bioinformatics

• Gene Symbol: LILRA5
• Uniprot:
  • A6NI73(Human)