IL6R Antibody (OTI2F4) - Azide and BSA Free

Western Blot: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6R.

Immunocytochemistry/Immunofluorescence: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL6R.

Immunohistochemistry: IL6R Antibody (OTI2F4) - Azide and BSA Free [NBP2-71029] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL6R Antibody (OTI2F4) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human IL6R(NP_000556) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IL6R Antibody (OTI2F4) - Azide and BSA Free

• CD126 antigen
• CD126
• gp80
• IL-6 receptor subunit alpha
• IL-6R 1
• IL-6R subunit alpha
• IL-6R-1
• IL6RA
• IL-6RA
• IL-6R-alpha
• interleukin 6 receptor alpha subunit
• interleukin 6 receptor
• interleukin-6 receptor subunit alpha
• Membrane glycoprotein 80
• MGC104991

Background

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL6R