IL6R Antibody (B-R6) - BSA Free

Flow Cytometry: IL6R Antibody (B-R6) [NB100-64770] - A surface stain was performed on human peripheral blood lymphocytes with IL6R (B-R6) antibody NB100-64770 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). A co-stain was performed with NBP2-24867AF488.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
• Clone: B-R6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL6R Antibody (B-R6) - BSA Free Summary

• Additional Information: Clone B-R6 was used by HLDA to establish CD designation.
• Immunogen: CHO cells transfected with cDNA of the IL-6 receptor
• Specificity: Recognizes soluble and membranous human IL-6R. This has been shown to inhibit IL-6 mediated proliferation of XG-1 cells, and partially blocks binding of IL-6 to its receptor. Removal of Sodium azide is recommended prior to use in functional assays.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6R
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL6R Antibody (B-R6) - BSA Free

• CD126 antigen
• CD126
• gp80
• IL-6 receptor subunit alpha
• IL-6R 1
• IL-6R subunit alpha
• IL-6R-1
• IL6RA
• IL-6RA
• IL-6R-alpha
• interleukin 6 receptor alpha subunit
• interleukin 6 receptor
• interleukin-6 receptor subunit alpha
• Membrane glycoprotein 80
• MGC104991

Background

IL6 exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa IL6 binding receptor protein (IL6R) and a 130 kDa signal transducing protein consisting of a 19 amino acid residue signal peptide (gp130). Human IL6R is a 468 amino acid residue type I membrane protein consisting of a 19 amino acid residue signal peptide, a 339 residue extracellular region, a 28 residue transmembrane region and an 82 residue cytoplasmic region. The ectodomain of IL6R is made up of an immunoglobulin like domain and a cytokine receptor superfamily domain. A naturally occurring soluble form of the IL6R, which binds IL6 and which mediates IL6 signaling through interaction with gp130, is identified in the urine of healthy adult humans. It has also been detected in human and mouse serum as well as in the conditioned medium of various cells including human peripheral blood mononuclear cells, and several T cell or granulocyte/macrophage cell lines. Increased expression of the c-fos and c-myc protooncogenes have been linked to IL6 receptor activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL6R
• Entrez:
  • 3570(Human)
• Uniprot:
  • P08887()