IL12B Antibody

Western Blot: IL12B Antibody [NB100-93409] - staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Primary incubation was

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IL12B Antibody Summary

• Description: Novus Biologicals Goat IL12B Antibody (NB100-93409) is a polyclonal antibody validated for use in WB and ELISA. Anti-IL12B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EARQQNKYENYS corresponding to internal region according to NP_032378.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL12B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in mouse spleen and rat liver lysates (calculated MW of 38.2 kDa band according to mouse NP_032378.1 and 38.4 kDa band according to rat NP_072133.1).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL12B Antibody

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12 subunit p40
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2IL12, subunit p40

Background

The IL12B gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encode

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL12B Antibody (NB100-93409)(1)

Publication using NB100-93409

• Stager S, Maroof A, Zubairi S et al. Distinct roles for IL-6 and IL-12p40 in mediating protection against Leishmaniadonovani and the expansion of IL-10+ CD4+ T cells. Eur J Immunol 2006-07-01 [PMID: 16791879]

Bioinformatics

• Gene Symbol: IL12B
• Entrez:
  • 16160(Mouse)
  • 64546(Rat)
• Uniprot:
  • P43432()