IL-7R alpha/CD127 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-7R alpha/CD127 Antibody [Biotin] Summary

• Immunogen:

  Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha

  
  Extracellular domain
• Specificity: Detects human IL-7 R alpha in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL7R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7R alpha/CD127 Antibody [Biotin]

• CD127 antigen
• CD127
• CD127ILRA
• CDW127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Background

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha . IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-/CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
3. Goodwin, R.G. et al. (1990) Cell 60:941.
4. Noguchi, M. et al. (1993) Science 262:1877.
5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
15. Jahn, T. et al. (2007) Blood 110:1840.
16. Lai, L. et al. (2006) Blood 107:1776.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7R alpha/CD127 Antibody (BAF306)(10)

Publications using BAF306

• Gaddiel Galarza-Muñoz, Debbie Kennedy-Boone, Geraldine Schott, Shelton S. Bradrick, Mariano A. Garcia-Blanco Antisense modulation of IL7R splicing to control sIL7R expression in human CD4+ T cells RNA 2022-08-01 [PMID: 35613883] (ELISA Detection, Human)
• P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes European Journal of Immunology, 2021-11-01;0(0):. 2021-11-01 [PMID: 34625948] (ELISA Detection, Human)
• SC Côté, SCB Schinkel, TK Berthoud, PO Barros, M Sanchez-Vi, AM Davidson, AM Crawley, JB Angel IL-7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection Eur. J. Immunol., 2020-05-27;0(0):. 2020-05-27 [PMID: 32390135] (ELISA Detection, Flow Cytometry, Human)
• H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele Nat Commun, 2019-10-08;10(1):4575. 2019-10-08 [PMID: 31594933] (ELISA Detection, Human)
• CJ Judge, L Kostadinov, KE Sherman, AA Butt, Y Falck-Ytte, NT Funderburg, AL Landay, MM Lederman, SF Sieg, JK Sandberg, DD Anthony CD56(bright) NK IL-7R? expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections J. Leukoc. Biol., 2017-04-11;0(0):. 2017-04-11 [PMID: 28400540] (ELISA Development, Human)
• Poiret , Thomas, Rane , Lalit, Remberger , Mats, Omazic , Birgitta, Gustafsson-Jernberg , Asa, Vudattu , Nalini K, Ahmed , Raija, Ernberg , Ingemar, Winiarski , Jacek, Magalhaes , Isabelle, Ringden , Olle, Maeurer , Markus Reduced plasma levels of soluble interleukin-7 receptor during graft-versus-host disease (GVHD) in children and adults. BMC Immunol, 2014-06-19;15(0):25. 2014-06-19 [PMID: 24946690] (ELISA Development (Detection), Human)
• Venet F, Foray A, Villars-Mechin A, Malcus C, Poitevin-Later F, Lepape A, Monneret G IL-7 Restores Lymphocyte Functions in Septic Patients. J Immunol, 2012-10-10;189(10):5073-81. 2012-10-10 [PMID: 23053510] (ELISA Development, Human)
• Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients. Genes Immun, 2012-08-23;13(7):587-92. 2012-08-23 [PMID: 22914435] (ELISA Development, Human)
• Hoe E, McKay FC, Schibeci SD, Gandhi K, Heard RN, Stewart GJ, Booth DR Functionally significant differences in expression of disease-associated IL-7 receptor alpha haplotypes in CD4 T cells and dendritic cells. J. Immunol., 2010-01-22;184(5):2512-7. 2010-01-22 [PMID: 20097866] (ELISA Development, Human)
• Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009-06-15;182(12):7389-97. 2009-06-15 [PMID: 19494261] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL7R